Leukotoxicity of Aggregatibacter actinomycetemcomitans in generalized aggressive periodontitis in Brazilians and their family members.

OBJECTIVE: The purpose of this study was to examine the leukotoxin promoter types of Aggregatibacter actinomycetemcomitans clones in subjects with generalized aggressive periodontitis (GAgP) and in their family members (FM). MATERIAL AND METHODS: Thirty-five patients with GAgP (33.9±7.1 years), 33 of their FM (22.8±11.4 years), and 41 patients with chronic periodontitis (CP) (44.1±9.4 years) were clinically analyzed using the plaque index, gingival index, probing depth (PD), and clinical attachment level (CAL). Subgingival biofilm samples were collected from four interproximal periodontal sites (>PD and >CAL) of each patient. The presence of A. actinomycetemcomitans and its leukotoxic clone was confirmed by polymerase chain reaction (PCR). RESULTS: A. actinomycetemcomitans was observed in 23 (51.1%) GAgP patients and 16 (30.1%) CP patients. Thirty-seven (94.8%) patients showed minimally leukotoxic strains and 2 (5.1%) showed highly leukotoxic strains. In the FM group, 10 (30.3%) had aggressive periodontitis (AgP), 12 (36.3%) had CP, 11 (33.3%) were periodontally healthy or had gingivitis, and 12.2% were A. actinomycetemcomitans positive. Greater full mouth PD and CAL were observed in GAgP patients positive for the bacteria than those negative for it (p<;0.05), and the presence of A. actinomycetemcomitans positively correlated with GAgP (Odds ratio, 3.1; confidence interval, 1.4-7.0; p=0.009). CONCLUSIONS: The presence of A. actinomycetemcomitans was associated with the clinical condition of GAgP, with most patients exhibiting a generalized form of the disease and minimally leukotoxic clones. Most of the relatives of GAgP patients presented either CP or AgP.

Leukotoxicity of Aggregatibacter actinomycetemcomitans in generalized aggressive periodontitis in Brazilians and their family members

OBJECTIVE: The purpose of this study was to examine the leukotoxin promoter types of Aggregatibacter actinomycetemcomitans clones in subjects with generalized aggressive periodontitis (GAgP) and in their family members (FM). MATERIAL AND METHODS: Thirty-five patients with GAgP (33.9±7.1 years), 33 of their FM (22.8±11.4 years), and 41 patients with chronic periodontitis (CP) (44.1±9.4 years) were clinically analyzed using the plaque index, gingival index, probing depth (PD), and clinical attachment level (CAL). Subgingival biofilm samples were collected from four interproximal periodontal sites (>PD and >CAL) of each patient. The presence of A. actinomycetemcomitans and its leukotoxic clone was confirmed by polymerase chain reaction (PCR). RESULTS: A. actinomycetemcomitans was observed in 23 (51.1%) GAgP patients and 16 (30.1%) CP patients. Thirty-seven (94.8%) patients showed minimally leukotoxic strains and 2 (5.1%) showed highly leukotoxic strains. In the FM group, 10 (30.3%) had aggressive periodontitis (AgP), 12 (36.3%) had CP, 11 (33.3%) were periodontally healthy or had gingivitis, and 12.2% were A. actinomycetemcomitans positive. Greater full mouth PD and CAL were observed in GAgP patients positive for the bacteria than those negative for it (p<;0.05), and the presence of A. actinomycetemcomitans positively correlated with GAgP (Odds ratio, 3.1; confidence interval, 1.4-7.0; p=0.009). CONCLUSIONS: The presence of A. actinomycetemcomitans was associated with the clinical condition of GAgP, with most patients exhibiting a generalized form of the disease and minimally leukotoxic clones. Most of the relatives of GAgP patients presented either CP or AgP. .

Genótipos fimA de porphyromonas gingivalis e periodontite / Porphyromonas gingivalis fimA genotypes and periodontitis

Porphyromonas gingivalis é um dos principais patógenos envolvidos com o início e progressão da doença periodontal. Este microorganismo exibe uma diversidade genotípica e fenotípica que pode resultar em diferenças na capacidade dos clones induzirem destruição periodontal. Variações no potencial patogênico dos distintos genótipos do gene fimA de P. gingivalis foram relacionadas a diferentes doenças periodontais em diversos estudos. Esta revisão teve o objetivo de analisar criticamente os estudos que relacionaram as condições periodontais e os diferentes genótipos fimA de P. gingivalis. Foram incluídas publicações na língua inglesa de estudos clínicos em humanos, que avaliaram a relação entre as condições periodontais e os diferentes genótipos fimA de P. gingivalis. Doze estudos foram considerados válidos para a realização desta revisão. Pôde-se concluir que os genótipos fimA II e IV de P. gingivalis encontram-se frequentemente associados à periodontite crônica

Porphyromonas gingivalis is a major pathogen involved in the initiation and progression of periodontal disease. This microorganism exhibits a genotypic and phenotypic diversity which may result in differences in the ability to induce periodontal destruction of the clones. Variations in the pathogenic potential of different genotypes of fimA gene of P. gingivalis were related to different periodontal diseases in several studies. This systematic review aimed to critically analyze the studies that have linked periodontal conditions and the different fimA genotypes of P. gingivalis. We included English language publications of human clinical studies that evaluated the relationship between periodontal conditions and the different fimA genotypes of P. gingivalis. Twelve studies were considered valid for the completion of this review. It was concluded that fimA genotypes II and IV of P. gingivalis are often associated with chronic periodontitis.